Our new Crystal clear+ tissue clearing system is the only technique that delipidates samples without any adjust in morphology and with negligible impact on structural integrity.
Megatome is usually a vibrating microtome designed to segment a broad variety of samples, from organoids and biopsy samples to expanded rodent brains and intact human organs. With significant blade vibrating frequency and minimized blade deflection, Megatome permits superior-throughput tissue sectioning with uniform area profile, as well as minimum tissue harm and data reduction.
Antibodies may possibly just take months to diffuse by way of just a few millimeters of tissue, by using a steep labeling gradient from floor to core.
Megatome is made for accuracy: the blade vibrates at a better frequency and larger amplitude assortment than other microtomes, and includes a distinctive deflection Manage mechanism.
Clone the undertaking or grab the latest release. Operating the utility will vary a tad according to how you retrieve the venture.
job to produce an aggregated Javadoc. Within this instance, javadocTask need to be established to allJavadoc to make sure that the correct documentation is designed ahead of making the docset.
You'll want to only should established the javadocTask residence once the endeavor you employ to make Javadoc is non-standard. One example is, there may be a endeavor named allJavadoc inside a multi-module
eFLASH is really a immediate tissue labeling strategy that allows for uniform total-organ staining in twenty rounds of labeling.
The SmartSPIM light-weight sheet microscope gives unparalleled resolution and acquisition velocity through intact tissue volumes, while its modular layout allows you to up grade and customize particular person components.
--icon: Specify an icon for use for the docset. Must be a 32x32 PNG, but this tool isn't going to confirm the file's information. No icon will probably be utilised if this is omitted.
This will likely make a docset named Sample in the current Listing. Docset generation may be tailored with optional arguments:
SHIELD avoids the variability of hydrogel embedding and the knowledge decline from PFA preservation, guarding specimens megatomi.com for a number of rounds of processing.
--displayName: Will established the name as proven in Dash. That is helpful if you develop a docset with name SampleProject but Show title Sample Job instead. This environment will default to the worth of --identify if omitted.
Our novel SHIELD tissue preservation approach kinds intramolecular bonds working with polyfunctional, adaptable epoxides to stabilize tissue architecture and safeguard the sample’s endogenous fluorescence, protein antigenicity and nucleic acids.